Were any animals harmed in the making of the BURST Floss? May 09, 2023 00:33 Updated No. The BURST Floss is not tested on animals. Related articles Why does the BURST Floss expand and how? Does the BURST Floss contain any PFA's? Is the BURST Floss biodegradable? Why are you removing charcoal from your brush heads and floss bobbins? Is the BURST Floss waxed?